Two-Dimensional Electrophoretic Analysis of Subcellular Liver Fractions and Isolated Hepatocytes from Normal and PFDA Treated Rats
Final rept. 15 Dec 1988-14 Dec 1989
INDIANA UNIV AT BLOOMINGTON DEPT OF BIOLOGY
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Perfluoro-n-decanoic acid PFDA effects on protein expression in the rat liver was studied following in vivo exposure to PFDAs LD50 and a lesser dose. Two-dimensional protein patterns were resolved on polyacrylamide gels for whole liver homogenate, mitochondrial, microsomal, cytosolic, and isolated hepatocyte protein fractions. PFDA exposure caused the deletion and induction of numerous proteins often in a dose related manner. Several proteins have been associated with those involved in lipid metabolism alterations characteristically induced by PFDA toxicity. Their actual identification awaits further study. The results of this project indicate that PFDA has significant effect on the quality and quantity of specific hepatocellular protein expression but whether these alterations are primary or secondary PFDA manifestations remains to be determined.