Accession Number:

ADA224751

Title:

Freshwater Cyanobacteria (Blue-Green Algae) Toxins: Isolation and Characterization

Descriptive Note:

Annual rept. (Final) 1 Nov 1986-30 Apr 1990

Corporate Author:

WRIGHT STATE UNIV DAYTON OH DEPT OF BIOLOGICAL SCIENCES

Report Date:

1990-05-01

Pagination or Media Count:

70.0

Abstract:

Freshwater cyanobacteria blue-green algae are capable of producing several physiologically distinct toxins. These toxins are produced by strains of the bloom-forming species Anabaena flos-aquae, Microcystis aeruginosa, Aphonizomenon flos-aquae, Oscillation agardhii and Nodularia spumingena. Work carried out under this contract covered the following areas with these biotoxin- producing microorganisms 1 Culture, using batch and semicontinuous culture methods, selected neuro- and hepatotoxin-producing strains of freshwater cyanobacteria. 2 Extract and purify the toxins using organic extraction followed by gel and ion-exchange column filtration both standard and high performance liquid chromatography HPLC. 3 Provide USAMRIID with selected purified hepatotoxic peptides and neurotoxic alkaloids. 4 Selected studies on optimization of culture conditions for toxic production and storage of toxic strains. 5 Continue collaborative studies in other areas of the U.S. and the world to isolate and compare freshwatermarine cyanobacteria toxins so that common methods of detectiondecontamination can be developed.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE