Freshwater Cyanobacteria (Blue-Green Algae) Toxins: Isolation and Characterization
Annual rept. (Final) 1 Nov 1986-30 Apr 1990
WRIGHT STATE UNIV DAYTON OH DEPT OF BIOLOGICAL SCIENCES
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Freshwater cyanobacteria blue-green algae are capable of producing several physiologically distinct toxins. These toxins are produced by strains of the bloom-forming species Anabaena flos-aquae, Microcystis aeruginosa, Aphonizomenon flos-aquae, Oscillation agardhii and Nodularia spumingena. Work carried out under this contract covered the following areas with these biotoxin- producing microorganisms 1 Culture, using batch and semicontinuous culture methods, selected neuro- and hepatotoxin-producing strains of freshwater cyanobacteria. 2 Extract and purify the toxins using organic extraction followed by gel and ion-exchange column filtration both standard and high performance liquid chromatography HPLC. 3 Provide USAMRIID with selected purified hepatotoxic peptides and neurotoxic alkaloids. 4 Selected studies on optimization of culture conditions for toxic production and storage of toxic strains. 5 Continue collaborative studies in other areas of the U.S. and the world to isolate and compare freshwatermarine cyanobacteria toxins so that common methods of detectiondecontamination can be developed.
- Medicine and Medical Research