Cloning Sequencing and Structural Manipulation of the Enterotoxin D and E Genes from Staphylococcus aureus
Final rept. 1 Nov 1985-30 Jun 1989
KANSAS STATE UNIV MANHATTAN DIV OF BIOLOGY
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We localized, cloned and sequenced the gene for staphylococcal enterotoxin D Sed. The gene was found on a large plasmid in all strains examined. Structurally, the gene is similar to the other enterotoxins, but bears closest similarity to SEA. Transcription of sed is regulated by the agr gene product and also is dependent upon spacing of the transcription start site from the translation start. We also cloned and sequenced the genes for the exfoliative toxins A and B. Eta was localized to the chromosome and Etb was identified on a large plasmid, similar to the Sed transcription of eta was dependent upon sequences near the 3 terminus of the gene. In addition, we have determined that the active site for exfoliative activity is localized to a 72 amino acid cyanogen bromide peptide at the C-terminus of the Eta molecule. Keywords Biotechnology, BW, Cloning, Enterotoxins, Sequencing, Staphylococcus aureus, Toxins, Lipase, Exfoliative toxin.