Toxic Potential of Nitroguanidine on Reproduction and Fertility in Rats. Volume 2. Part 2
Rept. for 8 Jul 1987-23 May 1988
LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA
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Nitroguanidine was mixed into the diet of Sprague-Dawley rats at 0, 1.3, 4.0, and 12.7 parts per thousandppt. In young adult rats these dose levels in ppt approximated the 100, 316, and 1000 mgkgday nitroguanidine dose levels in developmental toxicity studies in rats and rabbits. The diet was fed to parental males and females starting at 56 to 58 days of age and continued throughout their lives and to the F1 and F2 generation animals. Parental males and females were paired for mating. All matings were within the same dose group. Litters were examined and weighed at 0, 4, 7, 14, and 21 days of age. When pups were weaned at 21 days of age, 1 male and 1 female from each litter were selected to continue as parents for the next generation. Pups not selected and dams were euthanized. When the F1 animals were 20 weeks old the breeding procedure was repeated. The F2 pups and dams were euthanized at weaning. Nitroguanidine caused a decrease in some of the weekly body weights in the high dose animals, but the decrease was not consistent throughout the study. Terminal body weights were lower for the high dose F1 males and females and low dose F1 females. There were no dose-related effects on clinical signs, mating, fertility, gestation, litter size, pup weights or survival. Histopathological examination of the reproductive organs on adult animals and gross examination of weanlings showed no lesions attributable to nitroguanidine in any of the generations.