Use of Receptor Sites for Generic Detection of Chemical Agents and Toxins
Final rept. 15 Apr 1988-14 Apr 1990
TSI MASON RESEARCH INST WORCESTER MA BIOCHEMISTRY DEPT
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This project was an investigation of surface-bound matrices designed to resemble lipid bilayer membranes and to maintain functions of incorporated receptor proteins. A detergent dialysis technique was adapted to assemble membrane structures onto several solid supports. The incorporation and function of two types of membrane receptor proteins was studied. These were the visual receptor, rhodopsin, which functions by activating a G-protein cascade, and the nicotinic acetylcholine receptor, which is a ligand-gated ion channel. Data obtained on the composition, structure, and function of the surface-bound structures are consistent with the model of an anchored bilayer mimicking many of the attributes of a natural biomembrane. Keywords Biomembrane, Rhodopsin, Acetylcholine receptor, Membrane-mimetic, Membrane protein, Lipid, Biosensor.