Drug Retention during Animal Inhalation Exposure by FT-IR Spectroscopy
Technical rept. Jun-Aug 1988
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
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A unique analytical method for the determination of the amount of volatile drug retention by animals during inhalation exposure experiments was developed and evaluated. A large-volume collection system was used to collect and store all exhaled air from the animal. The collected air was analyzed by FT- IR spectroscopy utilizing a Nicolet 5SXB spectrometer and a Spectra-Tech, Inc. 10 meter long-path gas cell. The developed technique was evaluated using bis-trifluoromethyl disulfide TFD administered to adult sheep. Control experiments confirmed the systems ability to capture 100 of the administered compound. It was observed that the sheep retained an average of 4.4 mg of this compound per Kg of body weight during a 10-min exposure period at an air concentration of 5.0 mg per liter. After quantitative analysis, compounds exhaled by the animal were concentrated by passing the collected air through a series of dry ice and liquid nitrogen cooled traps. FT-IR analysis of the collected concentrated products indicated that TFD did not undergo chemical decomposition to form trifluoromethyl sulfide during the exposure experiment.