Accession Number:

ADA224444

Title:

Specific Radioisotopic Assay for Cholinesterase

Descriptive Note:

Technical rept. Dec 1987-Mar 1988

Corporate Author:

ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Report Date:

1990-05-01

Pagination or Media Count:

20.0

Abstract:

The radiometric method I for measuring ChE activity Siakotos et al., Biochem. Med. 3, 1, 1969 was modified to preclude the use of p-dioxane, a hazardous material. The modified procedure II uses 0.4 N perchloric acid PCA , instead of p-dioxane, to denature the ChE and stop hydrolysis of 14C- acetylcholine ACh. The unreacted substrate ACh is removed by cationic exchange resin suspended in water. The supernatant acidic water solution containing the product of hydrolysis, 14C-acetic acid, is mixed with nonhazardous scintillation cocktail and counted. The incubation mixture 37 degrees C for II is similar to I and is composed of 0.1 ml of buffer-salt solution pH 7.8, 0.1 ml of guinea pig whole blood WB-water suspension and 0. 1 ml of 3mM ACh solution. Keywords Acetylcholinesterase Acetylcholine Radiometric ChE assay Physostigmine Irreversible ChE inhibitors Titrametric ChE assay.

Subject Categories:

  • Biochemistry
  • Test Facilities, Equipment and Methods
  • Recording and Playback Devices

Distribution Statement:

APPROVED FOR PUBLIC RELEASE