Accession Number:

ADA224420

Title:

Immunotoxicological Assays Using the Japanese Medaka

Descriptive Note:

Annual rept.

Corporate Author:

MARYLAND UNIV SOLOMONS CHESAPEAKE BIOLOGY LAB

Personal Author(s):

Report Date:

1990-05-14

Pagination or Media Count:

24.0

Abstract:

Phagocytic blood cells secrete reactive oxygen intermediates ROI which are involved in immune defense reactions such as killing pathogenic microorganisms. The long-term goal of this project is to determine the effects of sublethal exposure to xenobiotics on ROI, and other immunological parameters, using a small aquarium fish model the Japanese medaka. A relevant blood cell model system Crassos trea virginica was used to develop lap assays for key ROI parameters superoxide anion, hydrogen peroxide, singlet oxygen, etc.. In addition to medaka studies, this was the first characterization of ROI in this species oyster. The application of ROI assays to fish goldfish, medaka, Fundulus produced encouraging preliminary results. Exposure of C. virginica blood cells to an environmental pollutant cadmium produced ROI inhibition, indicative of immunosuppression. The effects of in vivo and in vitro exposure of medaka macrophages to xenobiotics on ROI are under study. JES

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE