Neuroprotective Effects of Ketamine in a Rodent Model of Peptide Induced Spinal Cord Injury: Anatomical and Physiological Correlates
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF NEUROPSYCHIATRY
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Dynorphin A 1-17 D Y N is an endogenous peptide which produces spinal cord ischemia, nociceptive loss and hindlimb paralysis when injected into the lumbar cistern of unanesthetized or halothane anesthetized rats. We initially noted that ketamine K when used as the anesthetic agent during routine recording of somatosensory evoked potentials SEP preserved the SEP cortical response and affected the neurologic outcome in the previously reported paralytic dose 20 nM, DYN was used. Subsequently we reported that K and other N M D A receptor antagonists in the neurologic recovery following D Y N injection. The current study was divided into two experiments. The first, a neuroanatomical study divided into Groups I-IV, were performed in adult Sprague Dawley rats anesthetized with halothane. The i.t. dose of D Y N was 20 mm for all animals. A lumbar subarachnoid i.t. injection of saline I, D Y N alone 20 nM, II D Y N plus K 4nM, III and D Y N plus K 2nM, IV. The animals were neurologically evaluated for 72 hours, anesthetized and perfused per cardia with formalin and the lumbosacral cord removed.
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