Anapylactoid Reactions Mediated by Autoantibodies to Cholesterol in Miniature Pigs
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF BIOCHEMISTRY
Pagination or Media Count:
Autoantibodies to cholesterol were detected and purified from normal nonimmunized pig serum. The antibodies were assayed by enzyme-linked immunosorbent assay with crystalline cholesterol as an antigen and by complement-dependent damage to cholesterol-laden liposomes. Intravenous injection of liposomes containing cholesterol into anesthetized animals caused decreased hemolytic complement titers, and induced a reaction consisting of transient neutropenia, thrombocytopenia, respiratory distress, cyanosis, pulmonary and systemic hypertension, and decreased cardiac output. Plasma levels of thromboxane B2 and 6-keto-prostaglandin F1a increased 1300 and 200, respectively, and leukocyte and platelet counts decreased by 36 and 38 respectively. Injection of cholesterol-free liposomes did not induce the reaction.