Accession Number:

ADA224018

Title:

Differences in Conformational Stability Between Native and Phosphorylated Acetycholinesterase as Evidenced by a Monoclonal Antibody

Descriptive Note:

Corporate Author:

WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF BIOCHEMISTRY

Personal Author(s):

• Ashani, Yacov
• Gentry, Mary K.
• Doctor, Bhupendra P.

Report Date:

1990-03-13

Pagination or Media Count:

10.0

Abstract:

Monoclonal antibody 25B1 generated against diisopropyl phosphorofluoridate-inhibited fetal bovine serum acetylcholinesterase has been extensively characterized with respect to its anticholinesterase properties. This antibody demonstrated considerably different properties from previously reported inhibitory antibodies raised against acetylcholinesterase in terms of the degree of inhibition 98, the high degree of specificity, and the stability of the antigen-antibody complex. Keywords Acetylcholinesterase, Monoclonal antibodies, Organophosphates.

Descriptors:

• *ACETYLCHOLINESTERASE
• ANTIBODIES
• CLONES
• MONOCLONAL ANTIBODIES
• ORGANOPHOSPHATES
• CHOLINESTERASE INHIBITORS
• ANTIGENS
• REPRINTS
• INHIBITION

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE