Accession Number:

ADA222784

Title:

Automated Assays for Superoxide Dismutase, Catalase, Glutathione Peroxidase, and Glutathione Reductase Activity

Descriptive Note:

Corporate Author:

LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Report Date:

1990-01-01

Pagination or Media Count:

7.0

Abstract:

Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of nicotinamide adenine dinucleotide following the reduction of t-butyl hydroperoxide and oxidized glutathione, respectively. The assay for superoxide dismutase is based on the reduction of cytochrome c. All assays utilize the Cobras FARA clinical automated analyzer and provide considerable time savings over the manual assays. Reprints. jg

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE