Mass-Screening of Curarimimetic Neurotoxin Antagonists
Annual rept. 1 Feb 1988-31 Jan 1989
STATE UNIV OF NEW YORK AT STONY BROOK
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The third year of the contract period was devoted to testing several ion flux protocols for the distinction between toxic and therapeutic blockers of curarimimetric neurotoxins. First a cell line suitable for acetylcholine receptor channel analysis was identified among several rodent and human lines the murine tumor line BC3-H1 was selected because of its high receptor content. After determining optimal culture conditions for this line attempts were made to miniaturize the flux assay we were able to perform 86Rb flux measurement in standard 24-well multititer plates. Keywords Neurotoxin Acetylcholine receptor Ion flux Antidote.