Coxiella Burnetti Vaccine Development: Lipopolysaccharide Structural Analysis
Midterm rept. 26 Sep 1988-25 Sep 1989
HARVARD SCHOOL OF PUBLIC HEALTH BOSTON MA
Pagination or Media Count:
This Midterm report details new analytical approaches developed for the determination of endotoxin structures at much improved sensitivity and specificity, and an application of those techniques to Coxiella burnetti LPS. Foremost in this methodology is a reducing-end reagent synthesized to provide femtomole 10-15 detection for 0-antigen oligosaccharides. A second development focuses on methodology to extract the details of linkage, branching, and sequence, by periodate oxidation, supercritical fluid chromatography, and mass spectrometry. These techniques combine to provide the elements of a global approach to oligosaccharide structure. The utility of supercritical fluid chromatography for a determination of Lipid-A from Salmonella minnesota has been demonstrated. Finally, the structural features of Lipid-A from Coxiella burnetii is described using a combination of these newly developed analytical techniques. Keywords Lipopolysaccharide Immunogen Coxiella burnetii.