Temperature-Induced Changes in Reversed-Phase Chromatographic Surfaces: C7 and C8 Monomeric Ligands
Technical rept. Jun 1989-May 1990
UTAH UNIV SALT LAKE CITY DEPT OF CHEMISTRY
Pagination or Media Count:
The temperature-dependent retention of phenol and resorcinol on a monomeric C8 stationary phase, preconditioned with acetonitrile and then contacted with water, is investigated. These results are compared with the surface polarity of C7 and c8 monomeric ligands under similar conditions, the using the fluorescence vibronic band ratios of sorbed pyrene as a probe. Unlike polymeric stationary phases in the same size range, the solute elution and surface polarity of monomeric ligands indicate no persistent retention of the preconditioning solvent nor a distinct transition temperature for its accelerated loss. The results indicate that monomeric stationary phases are amorphous surface structures which do not achieve the same degree of conformational order as polymeric ligands the results are consistent with previous studies of the shape selectivity of monomeric and polymeric phases.
- Polymer Chemistry