Accession Number:

ADA222278

Title:

Role of Protein Phosphorylation in the Regulation of Neuronal Sensitivity

Descriptive Note:

Progress rept. 1 Jul 1989-31 Mar 1990,

Corporate Author:

CITY UNIV OF NEW YORK

Personal Author(s):

Report Date:

1990-03-30

Pagination or Media Count:

8.0

Abstract:

The project reported here focuses on the regulation of neuronal sensitivity by a novel class of protein kinase an ecto-protein kinase which phosphorylates proteins at the cell surface by extracellular ATP. We proposed that the ecto-protein kinase that our previous studies have described in neuronal cells may play a significant role in the regulation of neuronal signal transduction systems by extracellular ATP, and by extention in mechanisms underlying synaptic plasticity. Our studies are designed to provide experimental evidence in support of this hypothesis. The progress we have made in this project during the period reported here includes the development of novel experimental paradigms for the determination of ecto-protein kinase and its substrates in cultured cells. We used these paradigms in the conclusive identification of the surface phosphoproteins in primary neurons cultured from embryonic brain and in PC12 cloned neuronal cells induced to differentiate by nerve growth factor NGF. We also determined which of these surface phosphoproteins are regulated by NGF and during neurodevelopment, and which should be at the focus of continued studies on the role of ecto kinase in synaptic plasticity. jg

Subject Categories:

  • Biochemistry
  • Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE