Mass-Screening of Curarimimetic Neurotoxin Antagonists
Final rept. 1 Feb 1986-31 Jan 1990
STATE UNIV OF NEW YORK AT STONY BROOK DEPT OF BIOCHEMISTRY
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A screening procedure for inhibitors of alpha-bungarotoxin binding to nicotinic acetylcholine receptors from Torpedo californica electric tissue was developed which combines the sample handling capacity of microtiter plate technology with the speed and simplicity of autoradiography the screening protocol is amenable to further automation by liquid handling robotic equipment. Additional assays are needed to distinguish between blockers of neurotoxin binding that are themselves toxic and those that have therapeutic value here research focused on identifying a suitable cell line and on adapting the use of microwell plates to isotope flux assays. Good results were obtained with BC3-H1 cells grown and assayed in 24-well plates. Keywords RA 1 Neurotoxin Acetylcholine receptor Ion flux Bioassay Antidotes.