Western Immunoblot Analysis of Twenty-One Snake Venoms from Three Snake Families
DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)
Pagination or Media Count:
Snake venom components antigenically similar to those found in Vipera russelli venom was investigated across a wide spectrum of species. A polyvalent antiserum against Vipera russelli venom was raised in rabbits, and used in Western immunoblotting to probe nitrocellulose filters that held SDS-PAGE-size- separated snake venom proteins from twenty one different species, ranging from other Vipera spp. to Naja spp. It was found tht all species, even those distantly related to Vipera russelli, contained at least one immunologically cross-reacting component. The elapid samples were found to contain some of the strongest cross- reactive components, while many of the more closely related snakes showed weaker cross-reactivity. There appeared to be no correlation between the intensity and number of cross-reactive bands, and the relative evolutionary distance from Vipera russelli.