Monovalent Ligands of Complement Receptor 2 Inhibit Whereas Polyvalent Ligands Enhance Anti-Ig-Induced Human B Cell Intracytoplasmic Free Calcium Concentration
NAVAL MEDICAL RESEARCH INST BETHESDA MD
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We have performed experiments to investigate the role of ligands for complement receptor 2 CR2 in human B cell activation. Flow microfluorimetry was used to assess changes in free intracytoplasmic calcium concentration Ca2 in indo-loaded B cells, immediately after exposure to anti-micron antibody and to monovalent or polyvalent CR2 ligands. As monovalent ligands we used the C3d fragment and synthetic C3 peptides peptides P14, residues 1201-1214, and P28, residues 1187-1214. As polyvalent ligands we used i an intact monoclonal mouse anti-CR2 antibody HB5 and its Fab2 fragment, ii tetravalent P13 residues 1202-1214 4-template, and iii P28 conjugated to BSA molar ratio 51. B cell, Complement receptor 2, Antigen receptor, Cell calcium.
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