Accession Number:

ADA218380

Title:

Structure and Characterization of the Ion-Channel of the Nicotinic Acetylcholine Receptor

Descriptive Note:

Annual rept. 1 Jul 1982-30 Jun 1984,

Corporate Author:

CALIFORNIA INST OF TECH PASADENA

Personal Author(s):

Report Date:

1984-07-01

Pagination or Media Count:

31.0

Abstract:

The nicotinic acetylcholine receptor AcChR is a complex of four polypeptides of molecular weight 40,000, 50,000, 60,000 and 65,000 daltons, generally referred to as alpha, Beta, gamma, sigma, which we first described 1 and this has since been confirmed in several laboratories 2. By performing amino-terminal amino acid sequence analysis of these four polypeptides we demonstrated that they are a highly homologous set of proteins 3 presumably derived from a single ancestral gene and further that they are associated with a stoichiometry of alpha, Beta, gamma, sigma in AcChR from the electric ray, Torpedo californica, the most convenient source of this receptor molecule. Based on the amino acid sequence data we obtained, four groups constructed nucleotide probes for the isolation of C-DNA for subsequent sequence determination. kt

Subject Categories:

  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE