Accession Number:

ADA218357

Title:

Resistance to the Sterne Strain of B. anthracis: Phagocytic Cell Responses of Resistant and Susceptible Mice

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1989-01-01

Pagination or Media Count:

21.0

Abstract:

Inflammatory responses were compared in vivo, and host phagocytic cell functions compared in vitro, of mice resistant CBAJ and susceptible A J to lethal infection with the Sterne strain of Bacillus anthracis. Polymorphonuclear leukocyte PMN and macrophage responses at the initial site of infection were slower in AJ mice than in CBAJ mice. Whereas in AJ mice, the number of PMN ultimately responding to infection was equal to, or greater than, that in CBAJ mice, fewer macrophages accumulated. AJ mice failed to clear relatively low doses of the organisms and died. In vitro, chemotactic responses to both serum- and bacteria-derived attractants were similar for macrophages from AJ and CBAJ mice but were reduced for PMN from AJ mice. PMN and macrophages from the two mouse strains phagocytosed and killed spores in vitro to a similar extent, although killing by AJ PMN could be blocked by prior uptake of large numbers of killed spores. Thus susceptibility to lethal infection with Sterne strain correlated with the delayed influx PMN and reduced accumulation macrophages of phagocytes at the initial site of infection, but not with defective in vitro uptake or killing of spores. Keywords Bacillus anthracis Sterne Mice Resistance biology Anthrax Neutrophils Macrophages Phagocytic leukocytes Reprints.

Subject Categories:

  • Biology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE