Accession Number:

ADA218038

Title:

Isolation of a Toxin from Venom of Wagler's Pit Viper Trimeresurus wagleri

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1990-01-31

Pagination or Media Count:

23.0

Abstract:

A lethal toxin was isolated from Trimeresurus wagleri venom by fast protein liquid chromatography molecular sieve and Biorex-70 cation exchange. The toxin had an Mr of approximately 9.5 kD, a pl of 9.6-9.9, did not exhibit subunit behavior in SDS-PAGE profiles and lacked phospholipase A, proteolytic, and hemolytic activity. The toxin had an i.p. LD50 of 0.072 mgkg. The lethal activity was found to represent less than 1 of the total venom protein, which was only 62-65 of crude venom. The toxin lacked antigenic identity with a number of representative neurotoxins and myotoxins. The crude venom shared at least one antigen with Crotalus scutulatus venom. This antigen was not Mojave toxin. The toxin appears symptomologically suggestive of a vasoactive peptide or neurotoxin with specific blocking activity upon respiratory innervation. sdw

Subject Categories:

  • Medicine and Medical Research
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE