Accession Number:

ADA218033

Title:

Observations on a Venom Neutralizing Albumin Enriched Fraction Isolated from Serum of the Northern Copperhead Agkistrodon Contortrix Mokasen

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1990-01-31

Pagination or Media Count:

31.0

Abstract:

Venom neutralizing capacity of serum from the northern copperhead Agkistrodon contortrix mokasen was studied. Crude serum neutralized the hemorrhagic, proteolytic and lethal activity of A. c. mokasen venom. The serum neutralized up to 2.5 Lethal dose, 50 of A. c. mokasen venom and up to 5 LD50 of A. piscivorus venom. The serum was antihemorrhagic and a potent inhibitor of venom proteases and trypsin. An albumin enriched fraction was isolated from A. c. mokasen serum by DEAE-Affi-Gel blue chromatography and Superrose-12 molecular sieve fast protein liquid chromatography with recycling. This fraction contained two major proteins with apparent molecular weights of 62 kD and 79 kD respectively and several contaminants with an Mr from 52-79 kD. The fraction had a pl of 4.6-4.7 and displayed anodal migration in horizontal electrophoresis. jg

Subject Categories:

  • Biochemistry
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE