Accession Number:

ADA217714

Title:

Use of Monoclonal Antibodies to Study the Structural Basis of the Function of Nicotinic Acetylcholine Receptors on Electric Organ and Muscle and to Determine the Structure of Nicotinic Acetylcholine Receptors on Neurons

Descriptive Note:

Annual rept. (Final) 15 Feb 1989-30 Jun 1989

Corporate Author:

SALK INST FOR BIOLOGICAL STUDIES LA JOLLA CA RECEPTOR BIOLOGY LAB

Personal Author(s):

• Lindstrom, Jon M.

Report Date:

1989-09-30

Pagination or Media Count:

145.0

Abstract:

Extensive progress has been made on studies of the structure of nicotinic acetylcholine receptors from muscles and nerves. This has provided altogether new insights into the structure of neuronal nicotinic receptors Muscle-type receptors from Torpedo electric organs were shown to be composed of subunits organized like barrels staves around a central cation channel, in the order alpha beta alpha gamma sigma. Specific amino acid sequence were mapped topographically and to various functional domains. Xenopus oocytes were found to be a good expression system for small amounts of receptor. A human cell line was shown to synthesize relatively large amounts of functional receptors composed of alpha, Beta, gamma, and sigma subunits, and to synthesize nearly equal amounts of unassembled, partially mature alpha subunits. cDNAs for alpha and sigma subunits were cloned and sequenced. Keywords Protein sequencing, Protein purification, Ligands, Monoclonal antibodies, Genes, Ion channels, Bungaro toxins.

Descriptors:

• *NERVE CELLS
• *MONOCLONAL ANTIBODIES
• *CHOLINERGIC NERVES
• *NEUROMUSCULAR TRANSMISSION
• IONS
• CATIONS
• HUMANS
• PROTEINS
• SEQUENCES
• PURIFICATION
• LIGANDS
• GENES
• CLONES
• MUSCLES
• EGGS
• CHANNELS
• FROGS
• TOXINS AND ANTITOXINS
• AMINO ACIDS
• ORGANS(ANATOMY)
• ACETYLCHOLINE
• CHEMORECEPTORS
• NICOTINE
• SHARKS

Subject Categories:

• Biochemistry
• Genetic Engineering and Molecular Biology
• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE