Effect of Bacterial Endotoxin and Interleukin-2 on Human Leu-11(+) NK cells: Ultrastructural and Functional Correlations
NAVAL MEDICAL RESEARCH INST BETHESDA MD
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Bacterial endotoxin lipopolysaccharide, LPS and interleukin 2 IK- 2 are known to stimulate NK cell mediated cytotoxicity against tumor cells. In the present report we sought to correlate the stimulatory effect of IL-2nd LPS on NK cells with ultrastructural changes which occurred as a result of such stimulation. Peripheral blood mononuclear cells PBMC were purified from healthy donors by a Ficoll-Hypaque density gradient technique. Leu-11 NK cells were isolated by flow microfluorometry using a monoclonal FITC conjugated anti- Leu-11a antibody and a FACS II cell sorter. The PBMC were incubated respectively with E. coli LPS or recombinant IL-2 rIL-2 for various time periods. Sorted Leu-11 NK cells were incubated with LPS for 24 hrs. The NK cytotoxicity contained within the PBMC and sorted LEU-11 cells was assessed by a 51 Cr release technique using K562 tumor cells are targets. Reprints.