Accession Number:

ADA214793

Title:

Development of an Assay for the Early Detection of Organ Specific Carcinogenesis by the Determination and Turnover of NAD(+) and Polyadenosine Diphosphoribose

Descriptive Note:

Final rept. 19 Sep 1978-30 Sep 1980

Corporate Author:

CALIFORNIA UNIV SAN FRANCISCO

Personal Author(s):

Report Date:

1980-10-28

Pagination or Media Count:

88.0

Abstract:

A hypothesis was proposed that predicts the possibility of developing a specific biochemical methodology capable of detecting early and specific signals in chromatin proteins that precede cellular toxicity or malignant transformation, caused by toxic agents or by other environmental factors e.g., radiation. This long-range aim has been approached the identification of covalent modification of chromatin proteins by a specific enzymatic process of ADP-ribosylations and poly ADP-ribosylation to be the most probable biochemical area that can signal toxic effects leading to either cellular damage or malignant transformation. The experimental basis of this hypotheses is a. the immunochemical and more recently chemical localization and determination of chromosomal non-histone proteins as specific nuclear acceptors of ADP-ribose and of its polymers b. by the detection of specific perturbations in ADP- ribosylations of non-histone proteins during early carcinogenesis, development and hormonal influences, biological areas that are recognized to express differentiated cellular processes. Since the signals obtained during early carcinogenesis, development and hormone actions are clearly distinguishable, there is a predictably high probability that highly specific macromolecular mechanisms will become identifiable during subsequent research. Keywords Actin, Biosynthesis, Hormones, Aldosterone, Enzyme inhibitors, Hypophysectomy, Adrenolectomy.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE