Accession Number:

ADA214493

Title:

Regulation of lux Genes in Vibrio fischeri: Control of Symbiosis-Related Gene Expression System in a Marine Bacterium

Descriptive Note:

Annual rept. 15 Aug 1988-15 Aug 1989

Corporate Author:

IOWA UNIV IOWA CITY DEPT OF MICROBIOLOGY

Personal Author(s):

Report Date:

1989-11-04

Pagination or Media Count:

5.0

Abstract:

The lux genes of Vibrio fischeri encode the ability of this marine bacterium to produce light. V. fischeri occurs at high density in specialized light-emitting organs of certain marine fish, where the light produced is used by the fish. V. fischeri is also found in seawater, where it exists as a member of the bacterioplankton. In the planktonic habitat light-production is not useful and in fact V. fischeri possesses a genetic control mechanism which enables light production when the bacteria exist in the symbiotic state but does not allow synthesis of the light-emitting system when V. fischeri is in the planktonic habitat. This regulatory phenomenon is termed auto induction, and the aim of this research is to fully elucidate the mechanism of auto induction. Specific objectives of this research effort include a structurefunction analysis of the sensory receptor the LuxR protein, purification of the LuxR protein and development of a defined in vitro assay for studying lux gene transcription. Keywords Maine bacteria Symbiosis genes Gene regulation Auto induction Luminescence Chemical communication.

Subject Categories:

  • Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE