Accession Number:

ADA214254

Title:

Role of Second Messengers in Ischemic Tissue Damage

Descriptive Note:

Annual rept. 1 Nov 1988-31 Oct 1989

Corporate Author:

STANFORD UNIV CA SCHOOL OF MEDICINE

Personal Author(s):

Report Date:

1989-11-01

Pagination or Media Count:

39.0

Abstract:

Loss of vascular integrity and the consequent extravasation of intravascular fluid and blood cells into previously ischemic areas are initial events leading to tissue injury. The mechanism by which this damage occurs in the microvessels is unknown. Long-term goals of this project are to understand the second messenger system that controls microvascular angiogenesis so that better methods to prevent and control this tissue damage can be developed. Techniques used in this project include phase contrast and Nomarski light microscopy electron microscopy digital imaging fluorescence microscopy immunocytochemistry of cyclic AMP and cyclic GMP determination of and changes in protein kinase C and protein kinase A activity radiochemical determination of inositol triphosphate synthesis and analysis of the protooncogenes c-myc, c-fos, c-sis and c-ras and their modulation during angiogenesis. This report covers the following main areas development of better in vitro models of angiogenesis studies of the role of cyclic AMP in angiogenesis the effect of ischemia on angiogenesis the effect of pH on ischemic angiogenesis and the role of protein kinase C in angiogenesis. Keywords Adenosine phosphates Guanosine monophosphate. EDC

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE