Mutagenic Responses of some Petroleum-Base Obscurants in the Ames Test
Technical rept. Oct 1986-Dec 1987
CHEMICAL RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD
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In the Ames test, five petroleum fractions diesel fuel DF 25 distilled, DF with approximately 6 tetraethoxysilane, Jet-A, 75 bottoms product and Fog Oil, and methylene chloride extractions of combusted DF were evaluated for their mutagenic potential. The five fractions were dissolved in ethanol containing 1 Tween 80. One hundred milligrams of the residue from combusted DF was extracted with 25 mL of methylene chloride for 24 hr. After that time, the methylene chloride was replaced with acetone by evaporation. Each of five materials was tested in the four standard tester strains TA97, TA98, TA100, and TA102, metabolically activated aroclor 1254 induced rat liver S9 and nonactivated. Five concentrations, ranging upwards to the approximate level of cytotoxicity, of each material were tested, and the results in each case were confirmed with a similar nonconcurrent test. Positive results were not demonstrated in any of the materials tested. Keywords Ames test Metabolic activation Mutagenicity Cytotoxicity Cancer Tetraethoxysilane Fog oil Jet-A fuel 75 bottoms product Combusted diesel fuel Diesel fuel 25 distilled Extracts.