Accession Number:

ADA213230

Title:

Altered Signal Transduction in Renal Cell Injury Following Hemorrhagic Shock or Anoxia

Descriptive Note:

Annual rept. Jul 1988-Jul 1989

Corporate Author:

MARYLAND UNIV BALTIMORE DEPT OF PATHOLOGY

Report Date:

1989-07-01

Pagination or Media Count:

166.0

Abstract:

Our objective in this project is to delineate the relationship between transmembrane signalling ion deregulation, e.g., Ca2i, Hi, and Mg2i cell injury inflammation and repair. Our laboratory has been engaged in this type of work for some years and have recently reviewed the subject Trump et al., 1984 Trump and Berezesky, 1985. The achievement of this objective will have important implications for the prevention, diagnosis, and treatment of shock, trauma, related cell injury, and cell death. This project is aimed at the delineation of the relationship between ion deregulation e.g., Ca2i, Hi, Nai, Ki, and Mg2i, cell injury, signal transduction, altered DNA synthesis, and gene expression as they relate to cell death, regeneration, inflammation, and repair in the rat and human proximal tubule epithelium PTE. Techniques utilized include electron microscopy phase and Nomarski light microscopy digital imaging fluorescence microscopy DIFM and video microscopy, image analysis, and photon counting fluorescent probes, e.g., BCECF, rhodamine 123, and propidium iodide assays of the PI pathway including IP3 and DAG assays of protein kinase C and measurements of mRNA modulation of c-fos,. c-myc, and c-jun. Keywords Ion deregulation Calcium PH Cell injury Signal transduction DNA synthesis Gene expression Proximal tubule epithelium Anoxia Hemorrhagic shock Ischemia. kt

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE