Electron Microscopy of Intracellular Protozoa
Annual rept. 15 Sep 1987-14 Sep 1988
CASE WESTERN RESERVE UNIV CLEVELAND OH INST OF PATHOLOGY
Pagination or Media Count:
The distribution of circumsporozoite protein within developing P. malariae oocysts and salivary gland sporozoites and within P. cynomolgi exoerythrocytic stages was examined by immunoelectron microscopy using protein-A gold and monoclonal antibodies. Gold label was detected on the inner surface of peripheral vacuoles during oocyst maturation and the plasma membrane of the sporoblast. Salivary gland sporozoites and budding sporozoites in mature oocysts were labeled uniformly on the outer surface of their plasma membranes. The surface of sporozoites that ruptures into midgut epithelial cells were entirely covered with gold labels. Our results indicate that CS protein are synthesized in the oocyst before sporozoite formation and CS proteins of salivary gland P. malariae sporozoites are derived from the oocyst.