Monocyte-Derived Interleukin 1: Effects on Aortic Contraction and Phosphatidylinositol Turnover
NAVAL MEDICAL RESEARCH INST BETHESDA MD
Pagination or Media Count:
Medium conditioned by silica-stimulated human peripheral blood monocytes expresses vascular suppressive activity. Rat aortic rings, after incubation in conditioned medium, exhibited compromised contraction to stimulation by norepinephrine NE. Maximal contraction and sensitivity were both reduced in comparison to contraction and sensitivity displayed by rings after incubation in control medium. A polyvalent antibody Ab against human neutralized the suppressive activity in conditioned medium. Rings incubated in conditioned medium containing Ab exhibited normal maximal contraction and a partial restoration of sensitivity to NE. In contrast, incubation of rings in control medium supplemented with recombinant human I1 1 resulted in a dose dependent suppression of aortic contraction to NE that was analogous to the defects induced by monocyte conditioned medium. No significant differences in NE-stimulated hydrolysis of phosphatidylinositol PI were present between rings incubated in Ab-treated or untreated contractile function and that the mechanism by which I1 1 induces vascular dysfunction cannot be demonstrated to involve inhibition of NE-PI turnover. Keywords Sepsis, Human, Rat, Vascular contraction, Phorbol.
- Medicine and Medical Research