Determination of the Oxime, 2-Hydroxyiminomethyl-3-Methyl-1-(2-(3- Methyl-3-Nitrobutyloxymethyl) Imidazolium Chloride in Plasma by HPLC
Rept. for 30 Jan 1987-16 Jul 1988
LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA
Pagination or Media Count:
An assay for the quantitation of the oxime, 2-Hydroxyiminomethyl-3- Methyl-1-2-3-Methyl-3-Nitrobutyloxymethyl Imidazolium Chloride, WR 255,737CD 467, in plasma using high performance liquid chromatography is described. The above oxime WR 255,737 was extracted from plasma by an ion-pairing liquid liquid method, and analyzed by normal phase HPLC and UV detection. After the addition of the internal standard, 1-1-3-Butynyloymethyl-2- hydroxyiminomethyl-3-methyl imidazolium chloride WR 254,416, the sample was extracted into methylene chloride and subsequently back-extracted into a solution of 0.001M tetrabutylammonium hydrogen sulfate. Flow rate was 1.2 mlmin and the retention times were 2.1 min for WR 254,416 and 2.9 min for WR 255,737. Accuracy was evaluated from the analysis of blind, spiked samples which gave a coefficient of variation of or 2.6. The recovery was greater than 84 interday variability was less than 7.8 intraday variability was less than 3. 3. Stability was determined for WR 255,737 in plasma at -15C and -80C. There was no degradation at either temperature for 23 weeks. Keywords Antidotes, Organophosphates, Poisoning, Toxicity.
- Organic Chemistry