Accession Number:

ADA210384

Title:

Spatial Relationships between Drug Binding Sites on the Surface of the Acetylcholine Receptor

Descriptive Note:

Annual rept.

Corporate Author:

CALIFORNIA UNIV RIVERSIDE

Personal Author(s):

• Johnson, David A.

Report Date:

1987-10-15

Pagination or Media Count:

108.0

Abstract:

This report is divided into three sections dealing with the development and use of fluorescent probes of the nicotinic acetylcholine receptor AcChR. Section I summarized the purification and characterization of four mono-fluorescein cobra a-toxin derivatives. Section II deals with the use of the about derivatives to define the approximate orientation of cobra a-toxin on the acetylcholine receptor. Section III summarizes our measurements of the intersite distances between the binding sites for acetylcholine and phencyclidine on the nicotinic acetylcholine receptor.

Descriptors:

• *NEUROCHEMICAL TRANSMISSION
• *CHOLINERGIC NERVES
• *DRUGS
• *TOXINS AND ANTITOXINS
• PURIFICATION
• BINDERS
• VENOMS
• SENSE ORGANS
• ISOCYANATES
• ACETYLCHOLINE
• ANESTHETICS
• COBRAS
• ORIENTATION(DIRECTION)
• FLUORESCENCE
• NICOTINE
• SPATIAL DISTRIBUTION
• PROBES

Subject Categories:

• Biochemistry
• Anatomy and Physiology
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE