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Vector Competence of Mosquitoes for Arboviruses

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Annual rept. 28 JUl 1987-27 Jul 1988

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Aedes aegypti mosquitos are very refractory to peroral infection by dengue 2 New Guinea C virus ID50s in excess of 7 Log10 PFUml ingested. Parenteral infection ID50s are slightly above 2 Log10 PFUml. Binding of western equine encephalomyelitis WEE virus to brush border fragments BBF from mesenteronal epithelial cells of susceptible WS Culex tarsalis was greatly in excess of that to refractory WR Cx, tarsalis and Culex pipiens. The binding was specific. There are an estimated 1.7-3.4 x 10 to the 6th power binding sitesmesenteronal epithelial cell and the affinity constant is 2.3 x 10 to the 11th powerM. Monoclonal antibodies were produced against the BBF of mesenteronal epithelial cells. Three hybridomas 13A5, 13C1, and 20C5 were subcloned and ascites fluid produced. The 13A5 and 20C5 hybridomas are IgG2b subtypes and 13C1 is an IgM. Further biochemical analysis of BBF proteins revealed that Cx. pipiens closely resembles WR Cx. tarsalis in its 2D electrophoretic pattern. Reversed phase high performance liquid chromatography did not separate the major proteins of interest. A congenic line of high WEE virus producing HVP Cx. tarsalis was derived from low WEE virus producing LVP Cx. tarsalis after 13 generations of backcrossing.

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  • Biology
  • Microbiology

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