Accession Number:

ADA210231

Title:

Cloning and Characterization of the RecA Gene of Aquaspirillum magnetotacticum

Descriptive Note:

Corporate Author:

SRI INTERNATIONAL MENLO PARK CA MOLECULAR BIOLOGY DEPT

Personal Author(s):

• Berson, Amy E.
• Hudson, Debra V.
• Waleh, Nahid S.

Report Date:

1988-01-01

Pagination or Media Count:

21.0

Abstract:

The recA gene of Aquaspirillum magnetotacticum has been isolated from a genomic library and introduced into a recA mutant strain of Escherichia coli K12. The cloned gene complemented both the recombination and DNA repair deficiency of the host and its protein product promoted the proteolytic cleavage of the LexA protein. A protein whose molecular weight is similar to that of the RecA protein of E. coli was associated with the cloned sequence.

Descriptors:

• *MAGNETIC PROPERTIES
• *GENES
• MOLECULAR WEIGHT
• DEOXYRIBONUCLEIC ACIDS
• MUTATIONS
• SEQUENCES
• REPAIR
• DEFICIENCIES
• STRAINS(BIOLOGY)
• CLONES
• RECOMBINATION REACTIONS
• GENETIC ENGINEERING
• PROTEINS
• LIBRARIES
• BACTERIA

Subject Categories:

• Genetic Engineering and Molecular Biology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE