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Studies of Infection and dissemination of Rift Valley Fever Virus in Mosquitoes

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Annual rept. 15 May 1987-14 May 1988

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We are engaged in a multimethod study of Rift Valley fever RVF virus in vector and hypothetical vector mosquitoes. Major results and conclusions include 1 The patterns of midgut infection, escape of virus from the midgut, and distribution of virus after entering the hemocoel in Aedes mcintoshi are similar to those in Culex pipiens. 2 Immunocytochemical evidence indicates that the follicular epithelia and eggs of Ae. mcintoshi can become infected by virus in the hemolymph. 3 Out of 1771 Ae. mcintoshi collected from dambos in Kenya, one specimen appeared to be RVF viral antigen-positive by plaque assay. 4 We have made progress in elucidating the histology and ultrastructure of the mosquito proventriculus and the finding of putative virions in the foregut epithelium of specimens with nondisseminated infections supports the idea that RVF virus can enter the foregut epithelium via cells at the foregut-midgut junction. 5 We have found evidence of specific binding of components of formalin-killed RVF virus vaccine and mosquito cell membrane preparations. Keywords Rift Valley fever Mosquitoes Infection Dissemination Mosquito tissue tropisms Infection escape barriers transovarial transmission Electron microscopy Avidin-biotin-peroxidase complex ABC technique Viral plaque assay Vector competence Arbovirus receptor molecules

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  • Medicine and Medical Research
  • Microbiology

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