Accession Number:

ADA210135

Title:

Confocal Scanning Fluorescence Microscopy: A New Method for Phagocytosis Research

Descriptive Note:

Journal article

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s):

• Hook, Gregory R.
• Odeyale, Charles O.

Report Date:

1989-01-01

Pagination or Media Count:

7.0

Abstract:

An important new method for phagocytosis research, confocal scanning fluorescence light microscopy CSFM, is demonstrated using fluorescent microspheres ingested by murine macrophages. CSFM, in combination with Nomarski differential interference contrast microscopy DIC, can resolve microspheres inside cells from mircospheres attached to the surface of cells. Further, combined CSFM and DIC images can quantitate phagocytosis by individual cells aggregated together. No other method offers these capabilities. A comparison of CSFM and conventional epifluorescence light microscopy EFM images shows that CSFM produces significantly higher-resolution images of microspheres than EFM, primarily because CSFM excludes the out-of-focus light airfacts of EFM. Reprints

Descriptors:

• *INGESTION(PHYSIOLOGY)
• *PHAGOCYTES
• *RETICULOENDOTHELIAL SYSTEM
• *MICROSPHERES
• INTERFERENCE
• LIGHT
• FLUORESCENCE
• CONTRAST
• REPRINTS
• MICROSCOPY

Subject Categories:

• Biology
• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE