Accession Number:

ADA210088

Title:

Iron-Associated Outer Membrane Proteins of Magnetic Bacteria

Descriptive Note:

Final rept. Jul 1985-Jan 1989

Corporate Author:

NEW HAMPSHIRE UNIV DURHAM DEPT OF MICROBIOLOGY

Personal Author(s):

• Blakemore, Richard P.

Report Date:

1989-06-16

Pagination or Media Count:

82.0

Abstract:

This project contributed to improved understanding of the growth, physiology and control of magnetite production by the magnetic bacterium Aquaspirillum magnetotacticum. As a result of the research performed under this contract it has become possible to i culture A. magnetotacticum to high biomass yields in microaerobic continuous culture, and to ii precisely regulate through reproducible control of culture parameters, the formation of membrane proteins and bacterial magnetosomes in vivo. This is a vital prerequisite to studies of gene expression and magnetosome biogenesis in this organism. In contrast to iron uptake by enteric bacteria, cell iron uptake was found to involve a non-specific active transport mechanism at low iron concentration. A hydroxamate siderophore was produced only with the culture iron at 10 micro moles, or greater. Keywords Magnetism Bacteria Outer membranes Periplasm Iron reductase Cytochrome Magnetosome Biological absorption Membranes biology.

Descriptors:

• *MAGNETITE
• ENTERIC BACTERIA
• MAGNETIC PROPERTIES
• MEMBRANES(BIOLOGY)
• PARAMETERS
• BACTERIA
• PROTEINS
• IRON
• PRODUCTION CONTROL
• TRANSPORT
• YIELD
• GENES
• PHYSIOLOGY
• EXTERNAL
• IN VIVO ANALYSIS
• CELLS(BIOLOGY)
• REPRODUCIBILITY
• CULTURE
• BIOLOGICAL ABSORPTION
• BIOMASS CONVERSION
• CONTROL
• MAGNETIC FIELDS

Subject Categories:

• Biology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE