Accession Number:

ADA210038

Title:

Production of Human Monoclonal Rheumatoid Factor Secreting Hybridomas Derived from Rheumatoid Synovial Cells

Descriptive Note:

Journal article

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1989-01-01

Pagination or Media Count:

4.0

Abstract:

Although the cellular arm of the immune system is undoubtedly important in the pathogenesis of rheumatoid arthritis RA, RA is an autoimmune extravascular immune complex disease involving synovium and interstitial tissues. The major autoantibody present is rheumatoid factor RF, a polyclonal autoantibody directed to the Fc portion of IgG. Several observations point to RF-IgG complexes and their subsequent activation of complement as being important in the pathogenesis of RA. RF are heterogeneous in many qualitative characteristics including complement activating properties, specificity and avidity. For example, exhaustive studies attempting to demonstrate unique antigenic determinants on IgG Fc towards which serum RF is directed have been inconclusive, although many RA serum RF react with an antigen in the Fc that binds staphylococcal protein A. Characterizing the molecular genetic basis of RF in RA could provide exciting new understanding of important genetic and triggering factors in RA. However, this has not been possible because of the polyclonality of RF in RA. Hybridoma technology should overcome these difficulties and allow more precise characterization of these polyclonal RF, but reports to date using peripheral blood lymphocytes PBL have described only limited success. We herein describe successful production of human monoclonal IgM RF mRF secreting hybridomas derived from rheumatoid synovial cells RSC. Reprints.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE