Accession Number:

ADA210036

Title:

Monocyte-Derived Interleukin 1: Effects on Norepinephrine-Simulated Aortic Contraction and Phosphoinositide Turnover

Descriptive Note:

Journal article

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1989-01-01

Pagination or Media Count:

18.0

Abstract:

Medium conditioned by silica-stimulated human peripheral blood monocytes express vascular suppressive activity. Rat aortic rings, after incubation in conditioned medium, exhibited comprised contraction to stimulation by norepinephrine NE. Maximal contraction 300 or - 51 mg tensionmg tissue and sensitivity -5.91 or - O.23 M log EC50 were both reduced in comparison to contraction and sensitivity displayed by rings after incubation in control medium. A polyvalent antibody Ab against human interleukin 1 Il-1 neutralized the suppressive activity in conditioned medium. Rings incubated in conditioned medium containing Ab exhibited normal maximal contraction and a partial restoration of sensitivity to NE. In contrast incubation of rings in control medium supplemented with recombinant human Il-1 resulted in a dose- dependent suppression of aortic contraction to NE that was analogous to the defects induced by monocyte-conditioned medium. No significant differences in NE-stimulated phosphoinositide hydrolysis were present between rings incubated in Ab-treated or untreated conditioned or control media. The data suggest that monocyte-derived Il-1 may have a significant influence on vascular contractile function and that the mechanism by which Il-1 induces vascular dysfunction cannot be demonstrated to involve inhibition of NE-stimulated phosphoinositide metabolism. Keywords Sepsis Polyvalent antibody Vascular contraction Phorbol Endotoxic shock Reprints.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE