Accession Number:

ADA209971

Title:

Protein Dynamics Studied with Resonantly Enhanced Quasi-Elastic Light

Descriptive Note:

Annual rept. Jul 1988-1989

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD

Personal Author(s):

Report Date:

1989-07-15

Pagination or Media Count:

9.0

Abstract:

In order to investigate and understand the dynamics of protein fluctuations, specifically the interaction between dynamical structure and function, we have started a program to use resonantly enhanced light scattering to probe the fluctuation spectrum. Here we report on a new technique, the use of a quartz microbalance to confirm the existence of motion within myoglobin at 5 MHz. We report on modelling the temperature rise for an absorbing sample with incident laser light. Finally, we give some preliminary results of quasi-elastic light scattering on samples of membrane fragments containing the bacteria Bacteriorhodopsin. Keywords Raman spectroscopy Chromophores Membranes biology Protein dynamics Light scattering Resonance enhancement Microbalance Hydration shell Thermal diffusion Purple Membrane.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Stress Physiology
  • Test Facilities, Equipment and Methods
  • Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE