Potential Vaccine for Anthrax
Annual rept. 1 Jun 1987-31 May 1988
LOUISVILLE UNIV KY
Pagination or Media Count:
B. anthracis Sterne cells were treated with monoclonal antibodies MAbs to protective antigen and subjected to the indirect-fluorescent antibody assays. Fluorescence was discernible throughout the surface of cells. Extraction of the cells with 8M urea or 1 SDS brought about a total loss of fluorescence. Pretreatment of the same cells with trypsin seemed to render the fluorescence somewhat stronger, with distinct patterns of granularity. Preincubation of the cells with soy bean agglutinin seemed to bring about a patchiness in the fluorescence distribution. Polysaccharide, Indirect fluorescence antibody assays, Polysaccharide-monoclonals, Immunolabelling-TEM, Western blots, periodate oxidation, Smith degradation. 13C-NMR.