Phylogenetic Analysis of Marine Picoplankton Using rRNA Sequences
Progress rept. 20 Jun 1988-20 Jun 1989
INDIANA UNIV AT BLOOMINGTON DEPT OF BIOLOGY
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The purpose of the program is to analyze phylogenetically the dominant constituents of marine picoplankton in selected Atlantic and Pacific Oceans oligotrophic sites using 16S ribosomal RNA gene cloning and sequencing procedures. Additional goals are the development of rRNA-based hybridization probes for the phylogenetic analysis of single cells and the continued accumulation of 16S rRNA reference sequences. Recent efforts have resulted in the establishment of a recombinant DNA library of Sargasso Sea and northern Pacific picoplankton, the development of fluorescence-based, phylogenetic group- specific hybridization probes, and a continuing phylogenetic survey of cultivated cyanobacteria. Keywords Phylogeny Taxonomy Marine picoplankton 16S rRNA phylogeny Recombinant DNA Nucleic acid hybridization Nucleotide sequence analysis.