Characterization of Methanogen Membrane Function: A Genetic Approach
Final rept. Aug 1986-Jul 1989
ILLINOIS UNIV AT URBANA DEPT OF MICROBIOLOGY
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Methanococcus voltae is a marine methanogen which derives its energy from reactions derived from the reduction of carbon dioxide by hydrogen. We are interested in elucidating basic principles of energetics in this archaebacterium and have focused on active transport, ion translocation pathways and ATP generating systems. We have made significant progress in the elucidation and characterization of a novel P-type ATPase in this organism. The enzyme has been purified and partially characterized and the formation of an acyl phosphate intermediate demonstrated in its catalytic sequence. We have discovered an active transport system for HS-CoM, a novel methanogen coenzyme involved in a terminal step of methanogenesis. Mutants which are altered in this active transport system have been isolated and characterized.
- Genetic Engineering and Molecular Biology