Accession Number:

ADA207813

Title:

Microvascular Physiologic and Anatomic Responses of the Guinea Pig to Experimental Arenavirus Infection

Descriptive Note:

Rept. for 1 Jul 1987-28 Feb 1989

Corporate Author:

VETERANS ADMINISTRATION MEDICAL CENTER TUCSON AZ RESEARCH SERVICE

Personal Author(s):

Report Date:

1989-03-25

Pagination or Media Count:

65.0

Abstract:

These studies complete control observations in Guinea Pig strain 13 in preparation to examine microcirculatory disturbances in Pichinde virus infection. Findings are Using lymph flux analysis from intestinal mesenteric lymphatics, protein reflection coefficient is .728 or - .018 SEM, and permeability-surface area product is 3.21 or - .29 ulmin100g. For future studies involving endothelial monolayer transport from infected GP, in vitro new methods demonstrate 1 confluent endothelial monolayers can be assessed functionally from fluorescence intensity patterns of solutes in diffusion chambers, 2 cell morphology is identical to in vivo structure, 3 restricted diffusion develops after three days, and 4 single pore fits to permeabilities are excellent. Using ethane production to mark free radical production, we see 1 it is independent of minute ventilation, 2 is nearly exclusively produced in lung, 3 is inhibited by superoxide dismutase and catalase, 4 is tightly coupled to dietary iron, and 5 and is closely joined to microvascular abnormalities including increased filtration coefficient and lung water. New intravital microscopic methods including infusion of latex beads, have demonstrated 1 stable images are achieved, 2 mesenteric microvessels normally do not leak dextran, 3 kinetics of Kupffer cell phagocytosis are measurable, and 4 topical tumor necrosis factor results in dextran leaks and blot hemorrhages. This may be important in the splanchnic pathogenesis of hemorrhagic fever. Keywords Pichinde virus, Protein transport, Microcirculation, Water transport, Arenaviruses, Microvascular, Hemorrhage, Shock pathology.

Subject Categories:

  • Anatomy and Physiology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE