Selectable Sets of Novel Proteins: Catalytic and other Properties.
Final rept. 1 Jul-30 Sep 88,
PENNSYLVANIA UNIV PHILADELPHIA DEPT OF BIOCHEMISTRY
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This proposal aimed at the development of mathematical, computer, recombinant DNA, selection and screening procedures to attain adaptive evolution of entirely novel peptides or fusion proteins with useful catalytic, ligand binding, structural or other features. Potential uses range from industrial catalysis to production of new drugs and vaccines. A broad purpose of the experimental effort is to obtain novel peptides which can mimic the biological effects of almost arbitrary signal molecules such as hormones, growth factors, even pathogenic antigens. Mathematical models were developed to explore rugged adaptive landscapes associated with protein evolution. The fundamental importance of this work includes analysis of the distribution of function in peptide space and opening the way towards a technology of applied molecular evolution. AW
- Genetic Engineering and Molecular Biology