Effect of Chemical Mutagens on Herpes Virus - Induced Cellular Transformation and Testing for Mutagenesis in Mouse Cells
Final rept. 1 Jun 1979-31 May 1980
BRIGHAM YOUNG UNIV PROVO UT DEPT OF MICROBIOLOGY
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Assays which detect quantitative increases of morphological transformation in 3T3 cells were employed to detect transformation by seven temperature sensitive mutants of herpes simplex type 2 virus. It was determined that all seven mutants caused transformation at the non-permissive temperature. Mutant A8 293 caused formation of the most foci an appeared to be a more sensitive indicator of transformation in comparative experiments 31 foci per million cells compared to four foci per million cells for ultraviolet-irradiated wild type virus. Mutagenesis experiments using THO cells as indicator cells suggested that even after metabolic activation of the test chemicals this cell system does not provide an adequately sensitive test for mutagenesis. In additional experiments, further information was obtained which showed enhancement of transformation by hydrazine and 1,2-dimethylhydrazine of cells exposed to irradiated virus. Significant enhancement occurred in these in vitro tests only when the cells were exposed to the chemical 24 hours prior to virus infection.
- Medicine and Medical Research