Accession Number:

ADA207116

Title:

Investigation of Stress Induced Alterations in Neutrophil Function

Descriptive Note:

Annual rept. Jan-Dec 1988

Corporate Author:

MERRIFIELD LABS INC VA DEPT OF MEDICAL PSYCHOLOGY

Personal Author(s):

Report Date:

1989-01-10

Pagination or Media Count:

12.0

Abstract:

This study involves measurement of neutrophil migration to FMLP, adherence, and oxidative burst capacity in medical students as a result of their participation in simulated field medical operations. Two studies have been completed, and findings from these studies suggest that the experience of the exercise was associated with decreases in neutrophil adherence but did not produce any changes in cell migration to a chemo-attractant or in ability to undergo oxidative bursts. However, several logistical and assay problems arose during these studies though these studies allowed us to resolve these problems the data from these first two studies must be viewed cautiously. The primary goal of our research is to investigate the effects of stress during and after a military medical field exercise on neutrophil functions. Neutrophils were evaluated for random migration and chemotaxis using N - formyl - methionyl - leucyl - phenylalanine FMLP as a stimulant at 1 X 10 to the -7 power M in a 48 well modified Boyden chaber. Keywords Polymorphonuclear granulocytes.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Stress Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE