Accession Number:

ADA202965

Title:

Isolation of Genomic Clone for human Cholinesterase

Descriptive Note:

Annual summary rept. 15 Nov 1986-15 Nov 1987

Corporate Author:

MICHIGAN UNIV ANN ARBOR DEPT OF PHARMACOLOGY

Personal Author(s):

Report Date:

1987-12-01

Pagination or Media Count:

40.0

Abstract:

The cDNA for human cholinesterase EC 3.1.1.8 was isolated from a human basal ganglia library. The clone has a total length of 2426 base pairs bp, of which 1722 bp code for the mature protein, 212 bp are on the 5 side of the coding sequence, and 492 bp are in the 3 untranslated region. The signal peptide contains 28 amino acids. The amino acid sequence deduced from the cDNA exactly matched the 574 amino acid sequence of the cholinesterase found in human plasma. Therefore, our cDNA represents cholinesterase EC 3.1.1.8 rather than acetylcholinesterase EC 3.1.1.7. It was concluded that the amino acid sequence of cholinesterase from two different human tissues, brain and serum, was identical. Hybridization of genomic DNA blots suggested that a single gene or very few genes, coded for cholinesterase. A genomic clone was isolated using CDNA as a probe. The genomic clone contained exons 1 and 2. There appear to be a minimum of four exons in the cholinesterase gene, and we are searching for other genomic clones containing exons 3 and 4.

Subject Categories:

  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE